Acquisition of Temporal HIF Transcriptional Activity Using a Secreted Luciferase Assay.
| Author | |
|---|---|
| Abstract |
:
Here we describe a simple method based on secreted luciferase driven by a hypoxia-inducible factor (HIF) response element (HRE) that allows the acquisition of dynamic and high-throughput data on HIF transcriptional activity during hypoxia and pharmacological activation of HIF. The sensitivity of the assay allows for the secreted luciferase to be consecutively sampled (as little as 1% of the total supernatant) over an extended time period, thus allowing the acquisition of time-resolved HIF transcriptional activity. |
| Year of Publication |
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0
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| Journal |
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Methods in molecular biology (Clifton, N.J.)
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| Volume |
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1742
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| Number of Pages |
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37-44
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| Date Published |
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2018
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| ISSN Number |
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1064-3745
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| URL |
:
https://dx.doi.org/10.1007/978-1-4939-7665-2_4
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| DOI |
:
10.1007/978-1-4939-7665-2_4
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| Short Title |
:
Methods Mol Biol
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| Download citation |
